urinary bladder epithelial cell line t24  (LGC Standards)

Journal: Pharmaceutics

Article Title: Microencapsulation of Enteric Bacteriophages in a pH-Responsive Solid Oral Dosage Formulation Using a Scalable Membrane Emulsification Process

doi: 10.3390/pharmaceutics11090475

Figure Lengend Snippet: Live and confocal microscopy images of E.coli strain EV36-RFP cells (red) treated with microencapsulated EK1F in the presence of epithelial cells (blue): ( a ) Controls without phages (i) 2 h, (ii) 3 h, and (iii) 5 h following incubation of epithelial cells with E. coli strain EV36; ( b ) (i) phage amplification of free phages and EK1F in the presence of E.coli strain EV36, and live microscopy images of EK1F treated samples at (ii) 3 h and (iii) 5 h following incubation of epithelial cells with E.coli strain EV36; ( c ) (i) control epithelial cells not exposed to E.coli strain EV36, (ii) damaged actin visible for epithelial cells exposed to EV36 with no phage treatment, and (iii) the condition of actin of epithelial cells exposed to E.coli strain EV36 and treated with EK1F.

Article Snippet: The human urinary bladder epithelial cell line (ATCC ® HTB-4 TM ) was acquired from LGC Standards (UK).

Techniques: Confocal Microscopy, Incubation, Amplification, Microscopy, Control